基于特征荧光光谱的铜绿微囊藻MC-LR的快速预测

大气与环境光学学报 ›› 2011, Vol. 6 ›› Issue (6): 443-449.

基于特征荧光光谱的铜绿微囊藻MC-LR的快速预测

段静波^1,2，花日茂¹，刘文清²，张玉钧²，王志刚²，肖雪²，王欢博²

1. 安徽农业大学资源与环境学院，安徽合肥 230001
2. 中国科学院安徽光学精密机械研究所中国科学院环境光学与技术重点实验室，安徽合肥　230031

出版日期:2011-11-28 发布日期:2011-11-18
作者简介:段静波（1986-），女，硕士，研究方向为水体有毒污染物监测等,（电话）0551-5593691（电子信箱）Email:jbduan@aiofm.ac.cn
基金资助:
国家高技术研究发展计划(863 计划) 项目(2009AA063005)、安徽省科技计划项目（08010301107）、国家重大科技专项（2009ZX07527-005）和安徽省自然科学基金（11040606M26）资助

Rapid Estimation on Microcystin-LR Concentration Released by Microcystic Aeruginosa Using Feature Fluorescence Spectroscopy

DUAN Jing-bo，HUA Ri-mao, LIU Wen-qin, ZHANG Yu-jun, WANG Zhi-gang, XIAO Xue, WANG Huan-bo

College of Resources and Environment, Anhui Agricultual University, Hefei 230001, China;
Key Laboratory of Environment Optics and Technology , Anhui Institute of Optics and Fine Mechanics , Chinese Academy of Sciences , Hefei　230031 , China

Published:2011-11-28 Online:2011-11-18

摘要/Abstract

摘要：

以自然水体为培养基对铜绿微囊藻（Microcystic aeruginosa）进行了为期95天的实验室培养。测量了铜绿微囊藻不同生长阶段培养水体的三维荧光光谱和同步扫描荧光光谱，并且同步分析了藻类叶绿素a浓度和胞外微囊藻毒素MC-LR浓度。结果表明：根据三维荧光光谱得到荧光信号A、信号X和信号C，分别代表类蛋白、海洋腐殖酸和类富里酸。对各荧光信号强度与细胞外微囊藻毒素MC-LR浓度以叶绿素a浓度进行归一化，发现二者在藻类衰亡期具有相同的变化趋势，且最大值出现的时间较为一致，两者表现出显著的线性相关性。因此，结合荧光光谱特征信号和叶绿素a浓度可以实现对藻毒素MC-LR浓度的快速预测。就实验方法而言，△λ=80nm的同步扫描荧光光谱法相对于三维荧光光谱法具有耗时少、测量简便等优点，可以完整反映荧光信号A、X和C的强度，更适合用于以藻毒素快速预测为目的的水体荧光光谱在线分析。

关键词: 微囊藻毒素, 三维荧光光谱, 同步扫描荧光光谱

Abstract:

Microcystic aeruginosa was cultivated in laboratory condition with nature water for about 95 days. Three dimensional excitation-emission (3DEEM) fluorescence spectroscopy and synchronous scan fluorescence spectroscopy of the culture water at different growth stages were measured, simultaneously the chlorophyll-a and extracellular MC-LR concentration were analyzed. The results show that fluorescence signal A, signal X and signal C could be observed obviously from 3DEEM fluorescence spectroscopy and synchronous scan fluorescence spectroscopy, which represent protein-like substance, marine humic-like acid and fulvic-like substance respectively. All the three fluorescence signals intensity and MC-LR concentration were normalized by chlorophyll-a concentration. In the decline phase, the normalized fluorescence signals(A, X, C) and normalized MC-LR concentration show the same dynamic laws and good linear correlation. The maximum values appeared at the same time. Therefore, MC-LR concentration can be estimated by the feature fluorescence signal (A, X, C) and chlorophyll-a concentration. Comparing to 3DEEM fluorescence spectroscopy, synchronous scans fluorescence spectroscopy (△ λ = 80 nm) is less time-consuming and can give out the entire feature fluorescence signal (A, X, C). It is suitable for real-time analysis of water fluorescent spectroscopy which can be used to rapidly estimate the MC-LR concentration.

Key words: microcystins, three-dimensional fluorescence spectroscopy, synchronous scan fluorescence spectroscopy

段静波，花日茂，刘文清，张玉钧，王志刚，肖雪，王欢博. 基于特征荧光光谱的铜绿微囊藻MC-LR的快速预测[J]. 大气与环境光学学报, 2011, 6(6): 443-449.

DUAN Jing-bo，HUA Ri-mao, LIU Wen-qin, ZHANG Yu-jun, WANG Zhi-gang, XIAO Xue, WAN. Rapid Estimation on Microcystin-LR Concentration Released by Microcystic Aeruginosa Using Feature Fluorescence Spectroscopy[J]. Journal of Atmospheric and Environmental Optics, 2011, 6(6): 443-449.

参考文献

[1] Zhang Jingping, Xiao Fugang. Analysis and Detection Technology of Microcystin [M]. 2nd ed. Beijing: Chemical Industry Publisher, 2009(in Chinese). 张敬平, 肖付刚. 微囊藻毒素分析检测技术 [M]. 第二版. 北京: 化学工业出版社, 2009.
[2] Yoshida T. Acute oral toxicity of microcystin-LR, ayanobacterial hepatoxin in mice [J]. Nat Toxins, 1997, 5(3): 91-95.
[3] Dong Chuanhui, Yu Shunzhang, Chen Gang, et al. Detection of microcystins in water samples came from four zones and a lake of Jiangsu Province [J]. Journal of Environment and Health, 1998, 15(3): 168-169(in Chinese). 董传辉, 俞顺章, 陈刚, 等. 江苏几个地区与某湖周围水厂不同类型水微囊藻毒素调查 [J]. 环境与健康杂志, 1998, 15(3): 168-169.
[4] Carmichael W W, Azevedo S M F O, An J S, et al. Human fatalities from cyanobacteria: chemical and biological evidence for cyanotoxins [J]. Environ. Health Perspect., 2001, 109(7): 663-68.
[5] Azevedo S M F O, Carmichael W W, Jochimsen E M, et al. Human intoxication by microcystins during renal dialysis treatment in Caruaru-Brazil [J]. Toxicology, 2002, 181/182: 441-446.
[6] Chorus I, Bartram J. World Health Organization, Toxin cyanobacteria in water: a guide to their public health consequences, monitoring and management [R]. London, UK; E and FN Spon, 1999.
[7] National standards of People's Republic of China. GB-T 5749-2006 standars for drinking water quality [S](in Chinese). 中华人民共和国国家标准. GB-T 5749-2006 生活饮用水卫生标准 [S].
[8] ISO10260 water quality; measurement of biochemical parameters; spectrometric determination of the chlorophyll-a concentration 1992 [S].
[9] National standards of People's Republic of China. GB-T 20466-2006 determination of microcystins in water
[S](in Chinese) 中华人民共和国国家标准. GB-T 20466-2006 水中微囊藻毒素的测定 [S].
[10] Chen W, Westerhoff P, Leenheer J A, et al. Fluorescence excitation-emission matrix regional integration to quantify spectra for dissolved organic matter [J]. Environ. Sci. Technol., 2003, 37: 5701-5710.
[11] Hera N, Amy G, Park H R, et al. Characterizing algogenic organic matter (AOM) and evaluating associated NF membrane fouling [J]. Water Res., 2004, 38(6): 1427-1438.
[12] Elliot S, Lead J R, Baker A. Characterization of the ?uorescence from freshwater plankton bacteria
[J]. Water Res., 2006, 40(10): 2075-2083.
[13] McKnight D M, Boyer E W, Westerhoff P K, et al. Spectro?uorometric characterization of dissolved organicmatter for indication of precursor organic material and aromaticity
[J]. Limnol. Oceanogr., 2001, 46(1): 38-48.