猪肉冷藏过程中顶空挥发性有机物快速检测研究

大气与环境光学学报 ›› 2012, Vol. ›› Issue (4): 276-.

猪肉冷藏过程中顶空挥发性有机物快速检测研究

志中华, 沈成银, 杨彬, 刘升, 黄超群, 王鸿梅, 储焰南

（中国科学院安徽光学精密机械研究所环境光谱学研究室，安徽合肥 230031）

收稿日期:2012-03-15 修回日期:2012-03-22 出版日期:2012-07-28 发布日期:2012-07-19
通讯作者: 志中华(1986-)，女，辽宁铁岭人，研究生，主要从事VOCs在线质谱检测及数据统计分析研究。 E-mail:aristocat@126.com
作者简介:志中华(1986-)，女，辽宁铁岭人，研究生，主要从事VOCs在线质谱检测及数据统计分析研究。
基金资助:
中国科学院仪器研制(Y2005015)、中国科学院知识创新工程重要方向项目(KGCX2-YW-917)、国家自然科学基金(20577049, 21107112)、安徽省自然科学基金项目(11040606Q60)资助

Rapid Detection of Volatile Organic Compounds in the Headspace of Pork During Cold Storage Using Proton Transfer Reaction Mass Spectrometry

ZHI Zhong-hua, SHEN Cheng-yin, YANG Bin, LIU Sheng, HUANG Chao-qun, WANG Hong-mei, CHU Yan-nan

(Laboratory of Environmental Spectroscopy, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China)

Received:2012-03-15 Revised:2012-03-22 Published:2012-07-28 Online:2012-07-19

摘要/Abstract

摘要：

为了研究猪瘦肉与肥肉、五花肉在冷藏过程中的挥发性有机物(volatile organic compounds, VOCs)特征，利用自主研制的质子转移反应质谱分别对三类猪肉在低温冷藏过程中产生的VOCs进行定时直接检测，得到了不同猪肉在新鲜时和变质后的VOCs质谱图以及冷藏过程中特征VOCs的变化趋势图。分析发现乙醇是三种新鲜猪肉的共同VOCs特征，而新鲜肥肉的特征是顶空存在乙醛，新鲜五花肉则具有二者的共同特征。冷藏过程中，三类肉的顶空VOCs在1~9日内均处于相对稳定阶段，之后变化的共同特征是乙醇等不含硫VOCs浓度迅速上升后下降，而二甲基二硫醚等含硫VOCs浓度开始上升，但各类猪肉顶空的VOCs浓度比率及变化快慢有较大区别。

关键词: 猪肉, 质子转移反应质谱, 挥发性有机物

Abstract:

To study volatile organic compounds (VOCs) characteristic in the headspace of lean pork, fatty pork and marble pork during cold storage, the independently developed proton transfer reaction mass spectrometry (PTR-MS) was applied to directly detect the VOCs in the headspace of the three types of pork during cold storage. Along with the mass spectra of these VOCs when pork was fresh and spoilt, the trend of characteristic VOCs during cold storage were obtained. The result is that ethanol is the common VOCs characteristic of three types of fresh pork, and acetaldehyde is the characteristics of fresh fatty pork. The VOCs in the headspace of three types of pork were in a relatively stable stage during early nine days. After that, the intensity of some sulfur-free VOCs as ethanol increased and then decreased and the sulf-VOCs as dimethyl disulfide increased. However, the abundance and the change speed of VOCs for each type of pork is quite different.

Key words: pork, proton transfer reaction mass spectrometry, volatile organic compounds

中图分类号:

X836

志中华沈成银杨彬刘升黄超群王鸿梅储焰南. 猪肉冷藏过程中顶空挥发性有机物快速检测研究[J]. 大气与环境光学学报, 2012, (4): 276-.

ZHI Zhong-Hua, SHEN Cheng-Yin, YANG Bin, LIU Sheng, HUANG Chao-Qun, WANG Hong-Mei, CHU Yan-Nan. Rapid Detection of Volatile Organic Compounds in the Headspace of Pork During Cold Storage Using Proton Transfer Reaction Mass Spectrometry[J]. Journal of Atmospheric and Environmental Optics, 2012, (4): 276-.

参考文献

[1] Xiao Hong, Xie Jing. Determination of volatile compounds of chilled pork based on SPME-GC-MS [J]. Food Science 2010, 31(20): 406-409(in Chinese).
肖虹, 谢晶. SPME-GC-MS法测定冷却肉的挥发性成分 [J]. 食品科学, 2010, 31(20): 406-409.
[2] Ahn D U, Jo C, Olson D G. Analysis of volatile components and the sensory characteristics of irradiated raw pork [J]. Meat Science, 2000, 54(3): 209-215.
[3] Xu Y, Cheung W, Winder C L, et al. VOC-based metabolic profiling for food spoilage detection with the application to detecting Salmonella typhimurium-contaminated pork [J]. Analytical and Bioanalytical Chemistry, 2010, 397(6): 2439-2449.
[4] Mayr D, Margesin R, Klingsbichel E, et al. Rapid detection of meat spoilage by measuring volatile organic compounds by using proton transfer reaction mass spectrometry [J]. Applied and Environmental Microbiology, 2003, 69(8): 4697-4705.
[5] Jin Shunping, Li Jianquan, Han Haiyan, et al. Proton transfer reaction mass Spectrometry for Online detection of trace volatile organic compounds [J]. Progress in Chemistry, 2007, 19(6): 996-1006(in Chinese).
金顺平，李建权，韩海燕, 等. 质子转移反应质谱在线检测痕量挥发性有机物 [J]. 化学进展, 2007, 19(6): 996-1006.
[6] Lindinger W, Hansel A, Jordan A. Proton-transfer-reaction mass spectrometry (PTR-MS): on-line monitoring of volatile organic compounds at pptv levels [J]. Chemical Society Reviews, 1998, 27(5): 347-354.
[7] Li Jianquan, Shen Chengyin, Wang Hongmei, et al. Development and validation of proton transfer reaction mass spectrometry [J]. Chinese Journal of Analytical Chemistry, 2008. 36(1): 132-136(in Chinese).
李建权, 沈成银, 王鸿梅, 等. 质子转移反应质谱建立与性能研究 [J]. 分析化学, 2008, 36(1): 132-136.
[8] Li Jianquan, Shen Chengyin, Wang Yujie, et al. Study of on-line organic pollutants measurement with chemical ionization mass spectrometry [J]. Journal of Atmospheric and Environmental Optics, 2008, 3(5): 363-368(in Chinese).
李建权, 沈成银, 王玉杰, 等. 挥发性有机污染物实时在线化学电离质谱测量方法研究 [J]. 大气与环境光学学报, 2008, 3(5): 363-368.
[9] Shen C Y, Li J Q, Han H Y, et al. Triacetone triperoxide detection using low reduced-field proton transfer reaction mass spectrometer [J]. International Journal of Mass Spectrometry, 2009, 285(1-2): 100-103.
[10] Shen C Y, Li J Q, Wang Y J, et al. Discrimination of isomers and isobars by varying the reduced-field across drift tube in proton-transfer-reaction mass spectrometry (PTR-MS) [J]. International Journal of Environmental Analytical Chemistry, 2012, 92(3): 289-301.
[11] Wang Y J, Shen C Y, Li J Q, et al. Thermal desorption extraction proton transfer reaction mass spectrometer (TDE-PTR-MS) for rapid determination of residual solvent and sterilant in disposable medical devices [J]. Journal of Pharmaceutical and Biomedical Analysis, 2011, 55(5): 1213-1217.
[12] Wang Y J, Han H Y, Shen C Y, et al. Control of solvent use in medical devices by proton transfer reaction mass spectrometry and ion molecule reaction mass spectrometry [J]. Journal of Pharmaceutical and Biomedical Analysis, 2009, 50(2): 252-256.
[13] Shen Chengyin, Li Jianquan, Wang Hongzhi, et al. The study of multi-reagent in proton transfer reaction mass spectrometry [J]. Chemical Journal of Chinese Universities, 2012, 33(2): 263-267(in Chinese)
沈成银, 李建权, 王宏志, 等. 多反应离子的质子转移反应质谱 [J]. 高等学校化学学报, 2012, 33(2): 263-267.
[14] Shen Chengyin, Li Jianquan, Wang Hongzhi, et al. Proton transfer reaction mass spectrometry for on-line detection of trace volatile organic compounds in breath [J]. Chinese Journal of Analytical Chemistry, 2012, 40(5): 773-777. (in Chinese)
沈成银, 李建权, 王宏志, 等. 呼气中痕量挥发性有机物的质子转移反应质谱在线检测研究 [J]. 分析化学, 2012. 40(5): 773-777.
[15] Ahn D U, Nam K C, Du M, et al. Effect of irradiation and packaging conditions after cooking on the formation of cholesterol and lipid oxidation products in meats during storage [J]. Meat Science, 2001, 57(4): 413-418.