基于m序列调制技术的叶绿素荧光检测系统设计

大气与环境光学学报 ›› 2018, Vol. 13 ›› Issue (2): 97-103.

基于m序列调制技术的叶绿素荧光检测系统设计

石朝毅, 高先和*, 卢军, 谷艳红

（合肥学院电子信息与电气工程系，安徽合肥 230601）

收稿日期:2018-01-29 修回日期:2018-02-06 出版日期:2018-03-28 发布日期:2018-04-15
作者简介:石朝毅（1986-），男，河北石家庄人，博士，讲师，主要从事光电检测技术方面的研究。
基金资助:
Supported by Natural Science Foundation of Anhui Province（安徽省自然科学基金, 1708085QD87）, Natural Science Foundation of Higher Education Institutions of Anhui Province（安徽省高校自然科学研究重点项目, KJ2017A530）, Scientific Research Foundation for the Talents of Hefei University（合肥学院人才科研基金，16-17RC22）

Design of Chlorophyll Fluorescence Detection System Based on m Sequence Modulation Technique

SHI Chaoyi, GAO Xianhe*, LU Jun, GU Yanhong

(Department of Electronic Information and Electrical Engineering, Hefei University, Hefei, 230601, China)

Received:2018-01-29 Revised:2018-02-06 Published:2018-03-28 Online:2018-04-15
Supported by:
Supported by Natural Science Foundation of Anhui Province（安徽省自然科学基金, 1708085QD87）, Natural Science Foundation of Higher Education Institutions of Anhui Province（安徽省高校自然科学研究重点项目, KJ2017A530）, Scientific Research Foundation for the Talents of Hefei University（合肥学院人才科研基金，16-17RC22）

摘要/Abstract

摘要：

针对藻类叶绿素荧光信号微弱难以准确检测的问题，提出了基于m序列调制技术的叶绿素荧光检测系统。分析了荧光检测电路的噪声特点，设计了基于T型电阻反馈网络的I/V转换电路和二级放大电路；根据m序列解调原理，设计了双极性转换电路、移相电路和基于AD630的荧光信号解调电路。将系统应用于蛋白核小球藻叶绿素荧光检测，结果表明，对于叶绿素浓度为10 µg/L的蛋白核小球藻，测量相对标准偏差为2.61%。在0~100 µg/L叶绿素浓度范围内，检测系统输出直流电压值与蛋白核小球藻叶绿素浓度之间的线性相关系数达到0.998。

关键词: 叶绿素荧光, m序列调制, 相对标准偏差, 线性响应

Abstract:

According to the problems in the detection of weak chlorophyll fluorescence, a chlorophyll fluorescence detection system based on m sequence modulation technology was proposed. On the basis of noise analysis of fluorescence detection circuit, I/V conversion circuit and secondary amplifier circuit that based on T-type resistance feedback network were designed. According to the principle of m sequence demodulation, a bipolar conversion circuit, a phase-shift circuit and a fluorescence signal demodulation circuit based on AD630 were designed. The system was applied to measure the chlorophyll fluorescence of chlorella pyrenoidosa. For a sample with a chlorophyll concentration of 10 µg/L, the relative standard deviation of measurement values was 2.61%. The linear correlation coefficient between the output DC voltage and chlorophyll concentration was 0.998 in a chlorophyll concentration range of 0~100 µg/L.

Key words: chlorophyll fluorescence, m sequence modulation, relative standard deviation, linear response

中图分类号:

TN16
X122

石朝毅高先和卢军谷艳红. 基于m序列调制技术的叶绿素荧光检测系统设计[J]. 大气与环境光学学报, 2018, 13(2): 97-103.

SHI Chao-Yi, GAO Xian-He, LU Jun, GU Yan-Hong. Design of Chlorophyll Fluorescence Detection System Based on m Sequence Modulation Technique[J]. Journal of Atmospheric and Environmental Optics, 2018, 13(2): 97-103.

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