光合抑制法水质毒性检测中藻类培养光照与温度实验分析

大气与环境光学学报 ›› 2023, Vol. 18 ›› Issue (2): 133-140.

光合抑制法水质毒性检测中藻类培养光照与温度实验分析

陈金计 1,3, 殷高方 1,3*, 赵南京 1,3*, 张小玲 2, 甘婷婷 1, 陈敏 1, 亓培龙 1, 丁志超 1, 谷梦园 1, 王翔 1, 董鸣 1, 华卉 1, 王璐 1

1 中国科学院合肥物质科学研究院安徽光学精密机械研究所, 中国科学院环境光学与技术重点实验室, 安徽合肥 230031; 2 安徽大学物质科学与信息技术研究院信息材料与智能感知安徽省实验室, 安徽合肥 230039; 3 合肥学院生物食品与环境学院, 安徽合肥 230601

收稿日期:2021-04-07 修回日期:2021-06-03 出版日期:2023-03-28 发布日期:2023-04-18
通讯作者: E-mail: gfvin@aiofm.ac.cn;E-mail: njzhao@aiofm.ac.cn E-mail:njzhao@aiofm.ac.cn
作者简介:陈金计 (1996- ), 安徽合肥人, 硕士研究生, 主要从事浮游藻类光生物反应器在线测控技术研究。E-mail: jinjichen@aiofm.ac.cn
基金资助:
安徽省科技重大专项 (202003a07020007), 安徽省杰出青年科学基金项目 (1908085J23), 国家自然科学基金项目 (61875207、61805254、 62005001), 中科院仪器设备功能开发技术创新项目 (Y93H3g1251)

Experimental analysis of illumination and temperature of algae culture in water toxicity detection by photosynthesis inhibition method

CHEN Jinji 1,3, YIN Gaofang 1,3*, ZHAO Nanjing 1,3*, ZHANG Xiaoling 2, GAN Tingting 1, CHEN Min 1, QI Peilong 1, DING Zhichao 1, GU Mengyuan 1, WANG Xiang 1, DONG Ming 1, HUA Hui 1, WANG Lu 1

1 Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, HFIPS,Chinese Academy of Sciences, Hefei 230031, China; 2 Anhui Laboratory of Information Materials and Intelligent Perception, Institute of Material Science and Information Technology, Anhui University, Hefei 230039, China; 3 College of Biological Food and Environment, Hefei University, Hefei 230601, China

Received:2021-04-07 Revised:2021-06-03 Published:2023-03-28 Online:2023-04-18
Contact: Nan-jing Nan-jingZHAO E-mail:njzhao@aiofm.ac.cn

摘要/Abstract

摘要： 浮游藻类作为单细胞生物，其光合活性外界胁迫作用响应灵敏，是水质综合毒性检测的良好受试生物，因此认识受试藻种光合活性状态对温度和光照等主要环境因子的响应规律，掌握有效控制受试藻种光合活性状态和浓度的培养条件，对水质综合毒性检测至关重要。以模式受试生物蛋白核小球藻为研究对象，研究了不同温度梯度和光照强度下蛋白核小球藻光合活性变化规律。研究结果显示：蛋白核小球藻在不同梯度光照下，藻种光合活性和藻种浓度的变化非常明显。低光照下 (75 μE、125 μE) 藻种平均光合活性在0.60 左右但藻种浓度基本不增加；中光照下 (175、225、300、375 μE) 藻种平均光合活性在0.57 左右且此时藻种浓度有明显增加，其中，实验最佳光照为375 μE；高光照下 (475 μE和600 μE) 藻种平均光合活性低于0.56 (初始活性)，会对藻种的生长状态产生胁迫。不同梯度温度下藻种光合活性和藻种浓度也有明显变化，中低温下 (5、15、25 ℃) 藻种平均光合活性在0.59 左右且藻种浓度随着温度的升高而增加，最佳温度为25℃；高温下 (30、35、40 ℃) 藻种光合活性迅速下降直至失活。研究结果表明可以通过控制光照和温度来控制浮游藻类的光合活性和生长速度，为在线水质综合毒性测量仪提供标准的受试藻样培养方式，从而给便携式水质综合毒性测量仪的研发奠定基础。

关键词: 浮游藻类, 光合活性, 叶绿素荧光, 环境因子

Abstract: Planktonic algae are single-celled organisms, and their photosynthetic activity responds sensitively to external stress, so they are good test organisms for the comprehensive toxicity test of water quality. Obtaining the response law of the photosynthetic activity state of the tested algae species to the main environmental factors such as temperature and light, and mastering the culture conditions that effectively control the photosynthetic activity state and concentration of the tested algae species are essential for the comprehensive toxicity detection of water quality. Taking the model test organism Chlorella pyrenoidosa as the research object, the changes of photosynthetic activity of Chlorella pyrenoidosa under different temperature gradients and light intensities were studied in this work. The results show that the changes of photosynthetic activity and concentration of the algae species under different gradient illuminations are very obvious. Under low light (75 μE, 125 μE), the average photosynthetic activity of algae species is about 0.60 while the concentration of algae species basically does not increase. Under medium light (175, 225, 300, 375 μE), the average photosynthetic activity of algae species is around 0.57 and the concentration of algae species has increased significantly at this time. Among them, the best lighting for the experiment is 375 μE. Under high light (475 μE and 600 μE), the average photosynthetic activity of the algae species is lower than 0.56 (initial activity), which can stress the growth state of the algae species. It is also shown that the photosynthetic activity and concentration of algae species also changes significantly under different gradient temperatures. At medium and low temperatures (5, 15, 25 °C), the average photosynthetic activity of algae species is about 0.59, the concentration of algae species increases with the increase of temperature, and the best temperature is 25 ° C. Under high temperature (30, 35, 40 ° C), the photosynthetic activity of the algae species declines rapidly until inactivation. It is indicated that the photosynthetic activity and growth rate of planktonic algae can be controlled by controlling light and temperature, which can provide a standard method for cultivating the tested algae samples for a online water quality comprehensive toxicity meter, thereby laying a foundation for the development of the portable water quality comprehensive toxicity meter.

Key words: planktonic algae, photosynthetic activity, chlorophyll fluorescence, environmental factors

中图分类号:

X853

陈金计, 殷高方, 赵南京, 张小玲, 甘婷婷, 陈敏, 亓培龙, 丁志超, 谷梦园, 王翔, 董鸣, 华卉, 王璐 . 光合抑制法水质毒性检测中藻类培养光照与温度实验分析[J]. 大气与环境光学学报, 2023, 18(2): 133-140.

CHEN Jinji , YIN Gaofang , ZHAO Nanjing , ZHANG Xiaoling , GAN Tingting , CHEN Min , QI Peilong , DING Zhichao , GU Mengyuan , WANG Xiang , DONG Ming , HUA Hui , WANG Lu . Experimental analysis of illumination and temperature of algae culture in water toxicity detection by photosynthesis inhibition method[J]. Journal of Atmospheric and Environmental Optics, 2023, 18(2): 133-140.

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