基于叶绿素荧光的浮游植物生长状态监测技术

大气与环境光学学报 ›› 2014, Vol. 9 ›› Issue (3): 201-209.

基于叶绿素荧光的浮游植物生长状态监测技术

刘晶 1,2, 刘文清2, 赵南京2*,张玉钧2, 马明俊2, 戴庞达2, 殷高方2,段静波2,余晓娅2, 方丽2, 甘婷婷2

(1 中国科学技术大学物理学院光学与光学工程系, 安徽合肥 230023;
2中国科学院环境光学与技术重点实验室中国科学院安徽光学精密机械研究所, 安徽合肥 230031)

收稿日期:2013-10-09 修回日期:2013-12-18 出版日期:2014-05-28 发布日期:2014-05-21
作者简介:刘晶（1988-），男，安徽省宿松县人，博士研究生，主要从事荧光技术在水质监测中的应用与设备研制方面的研究。
基金资助:
国家“863”计划资助项目（2009AA063005）、国家科技重大专项（2009ZX07420-008-05）、安徽省杰出青年科学基金（1108085J19）、安徽省自然科学基金（11040606M26）资助

Monitoring Technique of Phytoplankton Growth State Based on Chlorophyll Fluorescence

LIU Jing1,2, LIU Wen-Qing2 , ZHAO Nan-Jing2†, ZHANG Yu-Jun2, MA Ming-Jun2, DAI Pang-Da2, YIN Gao-Fang2, DUAN Jing-Bo2, YU Xiao-Ya2, FANG Li 2, GAN Ting-Ting2

(1 School of Physical Science, University of Science and Technology of China, Hefei 230026, China;
2 Key Lab. of Environmental Optics & Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031,China)

Received:2013-10-09 Revised:2013-12-18 Published:2014-05-28 Online:2014-05-21

摘要/Abstract

摘要：

浮游植物生长状态监测以叶绿素荧光为基础，利用叶绿素荧光技术可以获得有关光系统II的大量信息。以蓝藻和绿藻为主要研究对象，采用两个450 nm蓝光LED分别用于激发本底荧光Fo和使样本处于光适应状态，利用超高亮激光二级管作为饱和脉冲光源获得最大荧光Fm。利用自行搭建的系统测量正常光照培养和对照组放置于暗室浮游植物样品的光化学反应效率Yield，研究了不同光适应状态下的最大相对电子传递速率ETRmax、光能利用效率α和光损伤参数β,并记录了完整的荧光诱导曲线和光响应曲线，实现了浮游植物生长状态的测定。

关键词: 浮游植物, 叶绿素荧光, 光合作用, 原位监测

Abstract:

The monitoring of phytoplankton growth state was based on chlorophyll fluorescence, mainly by using the modulated fluorometer. The changes in PSII performance of the photosynthetic apparatus have been explored by applying chlorophyll fluorescence technique. Chlorophyll fluorescence of microcystis aeruginosa and chlorella vulgaris were measured by system developed in lab. 450 nm LED were selected as excitation light source, which was used to obtain fluorescence at pigment level and made light-adaptation. LD was used to supply saturation pulse which could interrupt photochemical reaction briefly. The photochemical reaction efficiency Yield was measured between phytoplankton cultivated in normal light and in control which placed in a darkroom. The maximum relative electron transport rate ETRmax tested under different light-adapted state, light use efficiency α and light damage parameter β were investigated, fluorescence induction curves and light response curves were measured, and the determination of phytoplankton growth state can be achieved.

Key words: phytoplankton, chlorophyll fluorescence, photosynthesis, monitoring in situ

中图分类号:

X122
X83

刘晶刘文清赵南京张玉钧马明俊戴庞达殷高方段静波余晓娅方丽甘婷婷. 基于叶绿素荧光的浮游植物生长状态监测技术[J]. 大气与环境光学学报, 2014, 9(3): 201-209.

LIU Jing, LIU Wen-Qing, ZHIAO Na-Jing, ZHANG Yu-Jun, MA MIng-Jun, DAI Pong-Da, YIN Gao-Fang, DUAN Jing-Bo, YU Xiao-Ya, FANG Li, GAN Ting-Ting. Monitoring Technique of Phytoplankton Growth State Based on Chlorophyll Fluorescence[J]. Journal of Atmospheric and Environmental Optics, 2014, 9(3): 201-209.

参考文献

[1] Suggett D J, Moore C M, Geider R J. Estimating Aquatic Productivity from Active Fluorescence Measurements [A]. // In Chlorophyll a Fluorescence in Aquatic Sciences: Methods and Applications [M]. Dordrecht, The Netherlands: Springer Science+Business Media B.V., 2011: 103-127.
[2] Beutler M, Wiltshire K H, Meyer B, et al. A fluorometric method for the differentiation of algal populations in vivo and in situ [J]. Photosynthesis Research, 2002, 72(1): 39-53.
[3]Baker N R. Chlorophyll fluorescence: a probe of photosynthesis in vivo [J]. Annu. Rev. Plant Biol., 2008, 59: 89-113.
[4] Wang Zhigang, Liu Wenqing, Zhang Yujun, et al. The phytoplankton classified measure based on excitation fluorescence spectra technique [J]. China Environmental Science, 2008, 28(4): 329-333(in Chinese).
王志刚, 刘文清, 张玉钧, 等. 基于激发荧光光谱的浮游植物分类测量方法 [J]. 中国环境科学, 2008, 28(4): 329-333.
[5] Wang Zhigang, Liu Wenqing, Zhang Yujun, et al. Research on in-vivo measurement of phytoplankton biomass by LED excitation source [J]. Journal of Atmospheric and Environmental Optics, 2008, 3(1): 36-41(in Chinese).
王志刚, 刘文清, 张玉钧, 等. LED激发光源的水体浮游植物浓度活体检测系统研究 [J]. 大气与环境光学学报, 2008, 3(1): 36-41.
[6] Su Rongguo, Hu Xupeng, Zhang Chuansong, et al. Differentiation of phytoplankton populations by in vivo fluorescence [J]. Journal of Tropical Oceanography, 2008, 27(5): 24-29(in Chinese).
苏荣国, 胡序朋, 张传松, 等. 基于活体荧光的藻类识别测定技术 [J]. 热带海洋学报, 2008, 27(5): 24-29.
[7] Eppley R W. Temperature and phytoplank to growth in the sea [J]. Fish. Bull., 1972, 70(4): 1063-1085.
[8] Thomann R V, Mueller J A. Principle of Surface Water Quality Modeling and Control [M]. New York: Haper & ROV, 1987: 69-78.
[9] Schreiber U, Klughammer C, Kolbowski J . High-end chlorophyll fluorescence analysis with the MULTI-COLOR-PAM. I. Various light qualities and their applications [J]. PAM Application Notes, 2011. 1: 1-19.
[10] Wu Xiaodong, Kong Fanxiang, Cao Huansheng, et al. In situ study on the photochemical vitality of overwintering phytoplankton [J]. Journal of Lake Science, 2007, 19(2): 139-145(in Chinese).
吴晓东, 孔繁翔, 曹焕生, 等. 越冬浮游植物光合作用活性的原位研究 [J]. 湖泊科学, 2007, 19(2): 139-145.
[11] Schreiber U, Schliwa U, Bilger W. Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer [J]. Photosynthesis Research, 1986, 10(1-2): 51-62.
[12] Schreiber U. Pulse-Amplitude (PAM) Fluorometry and Saturation Pulse Method [A]. In Chlorophyll Fluorescence: A Signature of Photosynthesis [M]. Dordrecht, the Netherlands: Kluwer Academic Publishers, 2004: 279-319.
[13] Maxwell K, Johnson G N. Chlorophyll fluorescence—a practical guide [J]. Journal of Experimental Botany, 2000, 51(345): 659-668.
[14] Schreiber U, Muller J F, Haugg A, et al. New type of dual-channel PAM chlorophyll fluorometer for highly sensitive water toxicity biotests [J]. Photosynthesis Research, 2002, 74(3): 317-330.
[15] Liu Jing, Liu Wenqing, Zhao Nanjing, et al. The method of phytoplankton photosynthesis activity measurement in-situ based on light induced fluorescence [J]. Spectroscopy and Spectral Analysis, 2013, 33(9): 2443-2447.
刘晶, 刘文清, 赵南京, 等. 基于光诱导荧光技术的浮游植物光合作用活性原位测量方法 [J]. 光谱学与光谱分析, 2013, 33(9): 2443-2447.