基于24析因设计的重金属胁迫蛋白核小球藻联合毒性研究

大气与环境光学学报 ›› 2015, Vol. 10 ›› Issue (5): 376-385.

基于2⁴析因设计的重金属胁迫蛋白核小球藻联合毒性研究

段静波, 张玉钧, 赵南京, 殷高方, 肖雪, 方丽, 刘文清

(中国科学院安徽光学精密机械研究所中国科学院环境光学与技术重点试验室，安徽合肥 230031)

出版日期:2015-09-28 发布日期:2015-09-11
通讯作者: 段静波（1986—），女，山西长治人，博士研究生，主要从事水体微生物光学检测方法研究。 E-mail:jbduan@aiofm.ac.cn
作者简介:段静波（1986—），女，山西长治人，博士研究生，主要从事水体微生物光学检测方法研究。
基金资助:
国家自然科学基金（61378041）,国家“863”计划（2013AA065502，2014AA06A509，2009AA063005）,安徽省杰出青年科学基金（1108085J19）,安徽省自然科学基金（1408085MD72）资助

Joint Toxicity of Multi-Heavy-Metal to Chlorella Pyrenoidosa Based on 24 Factorial Design

DUAN Jingbo, ZHANG Yujun, ZHAO Nanjing, YIN Gaofang, XIAO Xue, FANG Li, LIU Wenqing

(Key Laboratory of Environmental Optics and Technology , Anhui Institute of Optics and Fine Mechanics , Chinese Academy of Sciences , Hefei 230031, China)

Published:2015-09-28 Online:2015-09-11

摘要/Abstract

摘要：

设计实验研究多种重金属胁迫藻类的联合毒性，更有利于阐明真实环境中重金属与藻类的毒性作用过程和机理。采用叶绿素荧光分析技术结合24析因设计方法研究重金属Hg2+、Cd2+、Cu2+和Zn2+胁迫蛋白核小球藻的联合毒性作用，得到主效应Hg、Cd、Cu、Zn以及交互作用Hg*Cu、Hg*Zn和Hg*Cu*Zn对蛋白核小球藻潜在最大量子效率Fv/Fm具有显著性影响（Sig.<0.05）的结论；通过多元线性回归拟合得到联合重金属浓度与藻类光合活性抑制率间的剂量-效应关系，得到模型表达式：y[%]= -22.557+19.926Hg+5.282Cu+0.015Zn+0.029Hg*Zn-0.002Hg*Cu*Zn，并对联合重金属胁迫蛋白核小球藻的机理做出解释；通过对模型进行统计学检验和实验验证，证明该模型可用于实验室条件下已知种类重金属Hg2+、Cd2+、Cu2+、Zn2+胁迫蛋白核小球藻的联合毒性估计。

关键词: 蛋白核小球藻, 析因设计, 重金属联合毒性, 多元线性回归

Abstract:

Designing experiments to research joint toxicity of multi-heavy-metal to algal is beneficial to illuminate toxic process and mechanism of heavy metals stress algal in real environment. Chlorophyll fluorescence analysis technique and 2⁴ factorial design method were used to design and analyze joint toxicity of heavy metal Hg2+, Cd2+, Cu2+ and Zn2+ to chlorella pyrenoidosa. Results show that the main effects of Hg, Cd, Cu, Zn and interaction effects of Hg * Cu, Hg * Zn and Hg * Cu * Zn influence chlorella pyrenoidosa photosynthetic fluorescence parameters Fv/Fm significantly (Sig. <0.05). The relationship between the concentration of heavy metals and photosynthesis inhibition rate was established by using multiple linear regression method. The model is as follows:y[%]= -22.557+19.926Hg+5.282Cu+0.015Zn+0.029Hg*Zn-0.002Hg*Cu*Zn, and it can be used to account for mechanism of heavy metals stressed chlorella pyrenoidosa. To test and verify the model by statistical method and experiment, the results show that the model can be used for estimating the joint toxicity of Hg2+, Cd2+, Cu2+ and Zn2+ stress chlorella pyrenoidosa in laboratory conditions.

Key words: chlorella pyrenoidosa, factorial design, joint toxicity of heavy metals, multiple linear regression

中图分类号:

X524

段静波张玉钧赵南京殷高方肖雪方丽刘文清. 基于2⁴析因设计的重金属胁迫蛋白核小球藻联合毒性研究[J]. 大气与环境光学学报, 2015, 10(5): 376-385.

DUAN Jing-Bo, ZHANG Yu-Jun, ZHAO Na-Jing, YIN Gao-Fang, XIAO Xue, FANG Li, LIU Wen-Qing. Joint Toxicity of Multi-Heavy-Metal to Chlorella Pyrenoidosa Based on 24 Factorial Design[J]. Journal of Atmospheric and Environmental Optics, 2015, 10(5): 376-385.

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基于2⁴析因设计的重金属胁迫蛋白核小球藻联合毒性研究

Joint Toxicity of Multi-Heavy-Metal to Chlorella Pyrenoidosa Based on 24 Factorial Design

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