激光诱导荧光探测气溶胶装置的研制及实验研究

大气与环境光学学报 ›› 2018, Vol. 13 ›› Issue (2): 104-111.

激光诱导荧光探测气溶胶装置的研制及实验研究

白同正1,2, 王珍珠2,* , 刘东2, 谢晨波2, 王英俭1,2

（ 1 中国科学技术大学环境科学与光电技术学院，安徽合肥 230026；
2 中国科学院安徽光学精密机械研究所大气成分与光学重点实验室，安徽合肥230031）

收稿日期:2017-01-19 修回日期:2017-02-14 出版日期:2018-03-28 发布日期:2018-04-15
作者简介:白同正（1991-），男，安徽人，研究生，主要从事激光诱导荧光技术方面的研究。
基金资助:
Supported by National Natural Science Foundation of China(国家自然科学基金，41590871), Youth Innovation Promotion Association CAS (中科院青促会，2017482)

Development and Experiment Results of Laser Induced Fluorescence Aerosol Detection Device

BAI Tongzheng 1,2, WANG Zhenzhu 2,* , LIU Dong 2, XIE Chenbo 2, WANG Yingjian 1,2

(1 School of Environmental Optics and Technology, University of Science and Technology of China, Hefei 230026, China;
2 Key Laboratory of Atmospheric Composition and Optical Radiation, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China)

Received:2017-01-19 Revised:2017-02-14 Published:2018-03-28 Online:2018-04-15
Supported by:
Supported by National Natural Science Foundation of China(国家自然科学基金，41590871), Youth Innovation Promotion Association CAS (中科院青促会，2017482)

摘要/Abstract

摘要：

基于激光诱导荧光原理研制出一套激光诱导荧光探测气溶胶的装置。该装置采用Nd:YAG脉冲激光器产生355 nm波长脉冲激光，经过引导后进入样品池，激发出的荧光被系统收集后进入高分辨率光栅光谱仪，得到样品的荧光光谱图。选用还原型辅酶I（NADH）和核黄素两种典型的生物荧光物质对系统的性能进行测试，在355 nm激光激发下，获取了还原型辅酶I（NADH）、核黄素荧光光谱图；研究了样品浓度对于荧光光谱强度的影响，结果表明在低浓度范围荧光强度与浓度呈线性关系；考查了激光能量对荧光强度的影响，在一定能量范围荧光强度与激光能量呈线性关系，但当激光能量达到一定值后荧光强度会发生饱和。该装置所建立的生物性物质荧光数据库有利于了解和掌握生物荧光特性，对于进一步实现生物气溶胶远距离探测具有重要意义。

关键词: 激光技术, 荧光光谱, 激光诱导荧光, 气溶胶

Abstract:

Based on the principle of laser induced fluorescence, a laser induced fluorescence aerosol detection device is developed. The device uses the Nd:YAG pulsed laser in 355 nm wavelength, and the laser is guided to sample cell, then the fluorescence is collected into the high resolution grating spectrograph, finally the fluorescence spectrum is displayed. Two typical biological fluorescent substances reduced coenzyme I (NADH) and riboflavin are chosen as samples to test the performance of the device. Under the excitation of the laser in 355 nm wavelength, the reduced coenzyme I and riboflavin fluorescence spectrum are obtained. The influence of concentration on the fluorescence intensity of riboflavin is studied experimentally, and the results show that fluorescence intensity has a linear relation with the riboflavin concentration during the low concentration range. The influence of the laser intensity is also studied, the results show that fluorescence intensity changes linearly with laser power within the limits, but the fluorescence intensity saturation will happen when the laser power reaches a certain value. The biological fluorescent materials database given by the laser induced fluorescence method is helpful to understand and grasp the biological fluorescence characteristics and is of significance to remote sensing of biological aerosols.

Key words: laser technology, fluorescence spectrum, laser induced fluorescence, aerosol

中图分类号:

O561.3

白同正王珍珠刘东谢晨波王英俭. 激光诱导荧光探测气溶胶装置的研制及实验研究[J]. 大气与环境光学学报, 2018, 13(2): 104-111.

BAI Tong-Zheng, WANG Zhen-Zhu, LIU Dong, XIE Chen-Bo, WANG Ying-Jian. Development and Experiment Results of Laser Induced Fluorescence Aerosol Detection Device[J]. Journal of Atmospheric and Environmental Optics, 2018, 13(2): 104-111.

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