Rapid Estimation on Microcystin-LR Concentration Released by Microcystic Aeruginosa Using Feature Fluorescence Spectroscopy

Abstract

Abstract:

Microcystic aeruginosa was cultivated in laboratory condition with nature water for about 95 days. Three dimensional excitation-emission (3DEEM) fluorescence spectroscopy and synchronous scan fluorescence spectroscopy of the culture water at different growth stages were measured, simultaneously the chlorophyll-a and extracellular MC-LR concentration were analyzed. The results show that fluorescence signal A, signal X and signal C could be observed obviously from 3DEEM fluorescence spectroscopy and synchronous scan fluorescence spectroscopy, which represent protein-like substance, marine humic-like acid and fulvic-like substance respectively. All the three fluorescence signals intensity and MC-LR concentration were normalized by chlorophyll-a concentration. In the decline phase, the normalized fluorescence signals(A, X, C) and normalized MC-LR concentration show the same dynamic laws and good linear correlation. The maximum values appeared at the same time. Therefore, MC-LR concentration can be estimated by the feature fluorescence signal (A, X, C) and chlorophyll-a concentration. Comparing to 3DEEM fluorescence spectroscopy, synchronous scans fluorescence spectroscopy (△ λ = 80 nm) is less time-consuming and can give out the entire feature fluorescence signal (A, X, C). It is suitable for real-time analysis of water fluorescent spectroscopy which can be used to rapidly estimate the MC-LR concentration.

Key words: microcystins, three-dimensional fluorescence spectroscopy, synchronous scan fluorescence spectroscopy

DUAN Jing-bo，HUA Ri-mao, LIU Wen-qin, ZHANG Yu-jun, WANG Zhi-gang, XIAO Xue, WAN. Rapid Estimation on Microcystin-LR Concentration Released by Microcystic Aeruginosa Using Feature Fluorescence Spectroscopy[J]. Journal of Atmospheric and Environmental Optics, 2011, 6(6): 443-449.

References

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