High Resolution Temperature Control System of  Microfluidic Fluorescence PCR

Journal of Atmospheric and Environmental Optics ›› 2015, Vol. 10 ›› Issue (5): 417-424.

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High Resolution Temperature Control System of Microfluidic Fluorescence PCR

FAN Shuguang1, ZHU Ling1,2*, ZHAO Shumi1, ZHANG Yuelong1, ZHANG Long1,2, DENG Guoqing1,2, WANG An1, LIU Yong1,2*

(1 Anhui Provincial Engineering Technology Research Center for Biomedical Optical Instrument, Institute of Applied Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China;
2 Wanjiang Center for Development of Emerging Industrial Technology, Tongling 244000, China)

Received:2014-11-11 Revised:2014-12-04 Online:2015-09-28 Published:2015-09-11
Contact: 范书广（1989-），男，安徽阜阳人，研究生，主要研究方向为光学精密仪器的研发。 E-mail:zhul@aiofm.ac.cn

Abstract

Abstract:

In the analysis of high resolution melting (HRM) of microfluidic fluorescence PCR amplification products, fluorescence intensity has a sudden change with the delink of DNA chain, while whether the abrupt change can be detected is dependent on the accuracy of temperature control. A kind of multi threshold PID algorithm was put forward, and a temperature control system based on FPGA was designed to adapt to the analysis of high resolution melting. Based on the difference of the measurement value and the set value, the temperature change can be controlled accurately through changing the pulse-time ratio of pulse width modulation(PWM) wave and the control frequency of the algorithm from the FPGA circuit system. After temperature calibrations of the measurement system, the accuracy precedes 0.1℃ and the resolution achieves ±0.01℃. The result of HRM experiment shows that this system can detect the single base mutation of DNA sequence successfully, and the temperature control system can meet the design requirements

Key words: microfluidic, platinum resistance, multi threshold PID algorithm, pulse width modulation wave, high resolution melting

CLC Number:

R318.6

FAN Shu-Guang, ZHU Ling, ZHAO Shu-Mi, Zhang Yue-Long, ZHANG Long, DENG Guo-Qing, WANG An, LIU Yong. High Resolution Temperature Control System of Microfluidic Fluorescence PCR[J]. Journal of Atmospheric and Environmental Optics, 2015, 10(5): 417-424.

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