Design of Chlorophyll Fluorescence Detection System Based on m Sequence Modulation Technique

Journal of Atmospheric and Environmental Optics ›› 2018, Vol. 13 ›› Issue (2): 97-103.

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Design of Chlorophyll Fluorescence Detection System Based on m Sequence Modulation Technique

SHI Chaoyi, GAO Xianhe*, LU Jun, GU Yanhong

(Department of Electronic Information and Electrical Engineering, Hefei University, Hefei, 230601, China)

Received:2018-01-29 Revised:2018-02-06 Online:2018-03-28 Published:2018-04-15
Supported by:
Supported by Natural Science Foundation of Anhui Province（安徽省自然科学基金, 1708085QD87）, Natural Science Foundation of Higher Education Institutions of Anhui Province（安徽省高校自然科学研究重点项目, KJ2017A530）, Scientific Research Foundation for the Talents of Hefei University（合肥学院人才科研基金，16-17RC22）

Abstract

Abstract:

According to the problems in the detection of weak chlorophyll fluorescence, a chlorophyll fluorescence detection system based on m sequence modulation technology was proposed. On the basis of noise analysis of fluorescence detection circuit, I/V conversion circuit and secondary amplifier circuit that based on T-type resistance feedback network were designed. According to the principle of m sequence demodulation, a bipolar conversion circuit, a phase-shift circuit and a fluorescence signal demodulation circuit based on AD630 were designed. The system was applied to measure the chlorophyll fluorescence of chlorella pyrenoidosa. For a sample with a chlorophyll concentration of 10 µg/L, the relative standard deviation of measurement values was 2.61%. The linear correlation coefficient between the output DC voltage and chlorophyll concentration was 0.998 in a chlorophyll concentration range of 0~100 µg/L.

Key words: chlorophyll fluorescence, m sequence modulation, relative standard deviation, linear response

CLC Number:

TN16
X122

SHI Chao-Yi, GAO Xian-He, LU Jun, GU Yan-Hong. Design of Chlorophyll Fluorescence Detection System Based on m Sequence Modulation Technique[J]. Journal of Atmospheric and Environmental Optics, 2018, 13(2): 97-103.

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Design of Chlorophyll Fluorescence Detection System Based on m Sequence Modulation Technique

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